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Nanda, Rabindra K.
- A Stability-Indicating HPTLC Method for Estimation of Nadifloxacin in Topical Cream
Authors
1 Dr. D. Y. Patil Institute of Pharmaceutical Sciences and Research, Pimpri, Pune-411018, Maharahtra, IN
Source
Research Journal of Topical and Cosmetic Sciences, Vol 1, No 1 (2010), Pagination: 25-29Abstract
The present work describes a stability-indicating HPTLC method for analysis of nadifloxacin in topical cream. The separation was carried out on Merck precoated silica gel aluminium plate 60 F254 using Chloroform: Methanol: Ammonia (4.3: 4.3: 1.4 v/v/v) as mobile phase. The densitometric scanning was carried out at 296 nm. Response was found to be linear in the concentration range of 50-300 ng /band with correlation coefficients (r2 = 0.9979). The method was validated as per ICH guidelines. The method was successfully applied for the analysis of drugs in pharmaceutical formulation. Nadifloxacin was subjected to forced degradation by acid, alkali, neutral, oxidation, dry heat, wet heat, sunlight, UV light. The degradation products were well resolved from the pure drug with significantly different RF values.Keywords
Nadifloxacin, HPTLC, Validation, Stability Studies.- Simultaneous Spectrophotometric Methods for Estimation of Cefpodoxime Proxetil and Potassium Clavulanate in Pharmaceutical Dosage Form
Authors
1 Department of Pharmaceutical Chemistry, Dr. D.Y.Patil Institute of Pharmaceutical Sciences and Research, Pimpri, Pune-411 018, IN
Source
Asian Journal of Research in Chemistry, Vol 4, No 10 (2011), Pagination: 1600-1604Abstract
Three simple, precise, accurate and reproducible spectrophotometric methods have been developed for the simultaneous estimation of Cefpodoxime proxetil (Cef) and Potassium clavulanate (Pot.clav.) in combined tablet dosage forms. The first method involves determination using the simultaneous equation method, the sampling wavelengths selected are 233.0 nm and 312.0 nm for Cef and Pot.clav. respectively. The second method involves determination using the multicomponent mode of the UV visible spectrophotometer, the sampling wavelengths selected are 233.0 nm and 312.0 nm for Cef and Pot.clav. respectively. The third method involves determination using the Area under curve, the sampling wavelength intervals selected are 235.0-231.0 nm and 314.0-310.0 nm for Cef and Pot.clav. respectively. All the methods were found linear in the concentration range of 1.5-50 μg/ml for both Cef and Pot.clav. The results of the analysis were validated statistically and recovery studies were carried out as per ICH guidelines.
Keywords
Cefpodoxime Proxetil, Potassium Clavulanate, Simultaneous Equation Method, Multicomponent Mode of Analysis, Area under Curve Method.- Development and Validation of Stability Indicating HPTLC Method for Simultaneous Estimation of Domperidone Maleate and Naproxen Sodium in Pharmaceutical Formulations
Authors
1 Department of Pharmaceutical Chemistry, Dr. D.Y. Patil Institute of Pharmaceutical Sciences and Research, Pimpri, Pune 18, Maharashtra, IN